S. Bigirimana¹*, P. Barumbanze¹, R. Obonyo² and J.P. Legg^2,3

¹ Institut des Sciences Agronomiques du Burundi, Gitega, Burundi
² International Institute of Tropical Agriculture, Eastern and Southern Africa Regional Centre, Kampala, Uganda
³ Natural Resources Institute, Chatham Maritime, UK

*isabu-gitega@usan-bu.net

Accepted for publication 27/10/03

Cassava mosaic disease (CMD) is the most important constraint to cassava (Manihot esculenta) production in Africa. Since the 1990s, the importance of the disease has been greatly increased by the spread through East and Central Africa of a pandemic of unusually severe CMD (Legg, 1999), associated with the recombinant begomovirus, East African cassava mosaic virus – Uganda (EACMV-UG) (Zhou et al., 1997). Following reports of the spread of EACMV-UG to Rwanda (Legg et al., 2001), it became apparent that Burundi, to the south, was also threatened.

A survey of cassava plantings in Burundi was therefore conducted in May/June 2003 to assess the status of CMD and to identify begomoviruses present. Fifty-three fields were sampled in ten of the country’s 16 regions and CMD-diseased leaf samples collected in each field for virus diagnosis. Assessments were made of CMD incidence, severity (using the standard 1-5 scale), infection type (either cutting or whitefly-borne) and abundance of the whitefly vector, Bemisia tabaci. Viruses were diagnosed from leaf samples using both specific primer PCR (Zhou et al., 1997) and restriction digestion with EcoRV and MluI of near full length DNA-A fragments amplified using universal begomovirus primers (Briddon & Markham, 1994).

EACMV-UG, ACMV, EACMV and mixed ACMV+EACMV-UG infections were identified from 17, 34, 1 and 3 sites respectively. EACMV-UG occurred at all sites in the north-eastern regions of Muyinga and Kirundo (Fig. 1), was present at some sites in northern Gitega, Ngozi, Karuzi, Kayanza, Ruyigi and northern Rutana, but was not recorded from southern Gitega, southern Rutana, Muramvya or Bujumbura. The EACMV-UG affected regions of Muyingo and Kirundo were distinct from the others in having higher CMD incidence (79% vs. 42%), a greater proportion of whitefly-borne to cutting-borne infection (1.9 vs. 0.6), more severe symptoms (4.17 vs. 3.26) and a greater abundance of B. tabaci (4.9 vs. 1.0). This data set provides clear evidence for the rapid spread of severe EACMV-UG associated CMD in the north-eastern regions of Kirundo and Muyinga. The occurrence of EACMV-UG at sites in northern, central and eastern Burundi also suggests that similar changes in CMD epidemiology are likely to occur here in the near future. These results represent the first report of the expansion of the African CMD pandemic into Burundi.

Acknowledgement

The study was funded by a grant from the United States Agency for International Development’s Office for Foreign Disaster Assistance.

References

Briddon RW, Markham PG, 1994. Universal primers for the PCR amplification of dicot-infecting geminiviruses. Molecular Biotechnology 1, 202-5.

Legg JP, 1999. Emergence, spread and strategies for controlling the pandemic of cassava mosaic virus disease in east and central Africa. Crop Protection 18, 627-37.

Legg JP, Okao-Okuja G, Mayala R, Muhinyuza J-B, 2001. Spread into Rwanda of the severe cassava mosaic virus disease pandemic and associated Uganda variant of East African cassava mosaic virus (EACMV-Ug). Plant Pathology 50, 796.

Zhou X, Liu Y, Calvert L, Munoz C, Otim-Nape GW, Robinson DJ, Harrison BD. 1997. Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination. Journal of General Virology 78, 2101-2111.

The British Society for Plant Pathology