First Report of multiple inflorescence disease of Cirsium arvense and its association with a 16SrIII-B subgroup phytoplasmas in Serbia

D. Rančić1, S. Paltrinieri2, I. Toševski3*, R. Petanović1, B. Stevanović4 and A. Bertaccini2

1 Faculty of Agriculture, University of Belgrade, Serbia
2 DiSTA, Patologia Vegetale, University of Bologna, Italy
3 CABI Bioscience, Centre Switzerland, Delémont, Switzerland
4 Faculty of Biology, University of Belgrade, Serbia

*tosevski@eunet.yu

Accepted for publication 20/01/05

Multiple inflorescences on Cirsium arvense (Asteraceae) have always been attributed to the presence of eriophyid mites, since their first record in 1892 (Petanovic et al., 1997). Plants showing symptoms of varying intensity were recorded from more than ten sites in northern Serbia between 2001 and 2003. Affected plants show irregular growth, atypical branching with little leaves, shortened internodes, hardening of the green inflorescence and reduced vigour, as well as either chlorosis or reddish-brown discoloration of the leaves and stems. Plants that reached flowering could also have multiple inflorescences (Fig. 1). In the field the disease persists for years, increasing slowly around the original infection focus. Infected plants senesce prematurely, inhibiting both seed production and rhizome propagation, which markedly reduces the C. arvense population.

Figure 1: C. arvense plant with multiple inflorescence disease

DNA was extracted from 8 samples of fresh, leaf midribs and multiple inflorescences from affected plants, and from similar tissues of 4 plants without symptoms. This DNA was used as template in a nested PCR using universal phytoplasma primers P1/P7, followed by primers F1/B6 (band ~1700 bp) and then primers R16F2/R2 (Duduk et al., 2004). Subsequent RFLP analyses with HpaII, TruI and HhaI restriction endonucleases were carried out. All 8 samples from plants with symptoms were PCR positive. RFLP patterns from the R16F2/R2 amplicons being identical to those of clover yellow edge phytoplasma (subgroup 16SrIII-B) but different from the TruI and HpaII profiles obtained using a reference strain of peach X disease. These results were confirmed by using primers R16(III)F2/R1, which are specific for group 16SrIII phytoplasmas and which amplify an ~800 bp product (Lee et al., 1994).

This is the first report of multiple inflorescence disease of Cirsium in Serbia and of its association with phytoplasmas belonging to rRNA group 16SrIII-B. These phytoplasmas have recently been identified in plants with similar symptoms in Hungarian vineyards (Palermo et al., 2004) and in other herbaceous or woody host species.

References

Duduk B, Botti S, Ivanovi M, Krstić B, Dukić N, Bertaccini A, 2004. Identification of phytoplasmas associated with grapevine yellows in Serbia. Journal of Phytopathology 152, 575-579.

Lee I-M, Gundersen DE, Hammond RW, Davis RE, 1994. Use of mycoplasma-like organisms (MLO) group-specific oligonucleotide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant. Phytopathology 84, 559-566.

Palermo S, Elekes M, Botti S, Ember I, Alma A, Orosz A, Bertaccini A, Kölber M, 2004. Presence of stolbur phytoplasma in Cixiidae in Hungarian vineyards. Vitis 43, 201-204.

Petanović R, Boczek J, Stojnić B,1997. Taxonomy and bioecology of eriophyids (Acari: Eriophyoidea) associated with Canada thistle, Cirsium arvense (L.) Scop. Acarologia 38, 181-192.

The British Society for Plant Pathology