First report of a leaf blight disease of Chlorophytum borivilianum caused by Colletotrichum capsici in northern India

Abdul Sattar, Mansoor Alam*, Abdul-Khaliq, Ram Sajeevan Shukla and Suman Preet Singh Khanuja

Microbiology & Plant Protection Division, Central Institute of Medicinal & Aromatic Plants, P. O. CIMAP, Lucknow-226 015, India

*email2alam@rediffmail.com

Accepted for publication 19/05/05

Safed musli (moosli; Chlorophytum borivilianum) is a commercially important medicinal plant in India. Cultivation of this crop in Lucknow, Pantnagar and adjoining areas in northern India was severely affected by a leaf blight disease in 2003 and 2004. Initial symptoms were the appearance of minute, pin-head, circular, reddish-brown lesions on the leaves, which form longitudinal streaks along the midrib, veins and margin of the infected leaves. Severely infected leaves dry prematurely and plants fail to produce healthy fingers (tubers) used for medicinal purposes. The disease occurred in August and September, after the onset of the rainy season, and caused losses of up to 30% in severely affected fields.

Figure 1: Typical symptoms of leaf blight disease caused by Colletotrichum capsici on safed musli

Isolations from the young and mature necrotic lesions on potato dextrose agar (PDA) invariably yielded a Colletotrichum species. The fungus initially produced white to grey mycelial growth which became reddish brown due to copious sporulation after 5-7 days. Acervuli were numerous, globose to saucer shape with large number of dark brown setae 96-124 µm long. Conidiophores were short, simple and hyaline. Conidia were aseptate, fusiform, sickle shape and single celled. Those from host material were 10-15 (13) x 3-4 (3) µm and 10-21 (14) x 3-5 (4) µm after 7-10 days-growth on PDA. Based on these morphological features the fungus was identified as Colletotrichum capsici. Isolates were identical morphologically to Colletotrichum capsici isolates infecting Ocimum basilicum (Alam et al. 1981). To prove pathogenicity, 45-day old healthy plants, bearing 5-6 leaves, were sprayed with Colletotrichum capsici isolates from Lucknow and Pantnagar regions, in aqueous suspensions of 1.5 x 10⁶ conidia per ml. Symptoms identical to those found occurring naturally were observed 3-5 days after inoculation. After 7-12 days the original lesions had spread length-wise and often coalesced to form long necrotic streaks, producing typical leaf blight symptoms (Fig. 1). The inoculated plants died prematurely. Control plants sprayed only with water remained healthy. C. capsici was reisolated from the inoculated plants.

This is the first record of Colletotrichum capsici on safed musli from India. Colletotrichum chlorophytumi has been recorded on Chlorophytum spp. growing in pots in various gardens in Allahabad (Sudhir Chandra & Tandon, 1965). This species has slightly longer and broader conidia and infections resulted in leaf spots, which at maturity had three distinct zones of colour; a feature not seen with Colletotrichum capsici on Chlorophytum borivilianum.

References

Alam M, Janardhanan KK, Singh HN, Husain A, 1981. A new leaf blight of French basil caused by Colletotrichum capsici in India. Journal of Mycology and Plant Pathology 10, 99.

Sudhir Chandra, Tandon RN, 1965. Two new leaf spot fungi. Current Science 34, 565-566.

The British Society for Plant Pathology