EFFECT OF RALSTONIA SOLANACEARUM GLYCOPOLYMERS ON TOMATO DEVELOPMENT IN DISEASE RESISTANCE
VA MURAS, NV ZHITKEVICH, LD VARBANETS and RI GVOZDJAK
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 252143, 154 Zabolotny Str., Kyiv, Ukraine
Background and objectives
Materials and methods
Results and conclusions
Results showed that pre-sowing treatment of seeds gave better results compared with the control. Treatment of seeds for 6 h causes better and faster development of tomato plants, especially when the concentration of GP was 0.1 mg/ml. The ability of seeds to sprout was 15% higher, the quantity of tomato flowers 20% more, and of fruits 25% more, compared with control plants. Thus pre-sowing treatment of tomato seeds by GP solution affects the intensity of growth and development.
Tomato plants which were grown from seeds treated by GP solution were infected by R. solanacearum 9082, Erwinia carotovora 8982, Xanthomonas campestris pv. vesicatoria 9, and Clavibacter michiganensis subsp. michiganensis 9051. As a control we used tomato seeds treated with sterile water. We established the ability of GPs to increase tomato plant resistance with reference to subsequent infection by pathogens. Experimental plants infected by R. solanacearum were characterized by brown, necrotic (leaves) or oily (fruit) spots which were of lesser intensity and size compared with control plants. At the end of the experiment, the amount of fruit damaged by rot was greater in control plants. Analogous results were obtained when the plants were infected by X. campestris pv. vesicatoria. There were no tomato plants resistant to infection by Erwinia carotovora. Tested plants were completely resistant to infection by C. michiganensis subsp. michiganensis, the agent of bacterial canker disease. These findings may be used to increase tomato plant resistance and also as a basis for subsequent selective breeding investigations.