2.2.102
GENETIC DIVERSITY OF MELAMPSORA POPULATIONS DETECTED BY AFLP MARKERS

B SAMILSl, U LAGERCRANTZl, M LASCOUX2 and U GULLBERGl

lDepartment of Plant Biology, SLU, 750 07 Uppsala, Sweden; 2Department of Genetics, Uppsala University, 750 07 Uppsala, Sweden

Background and objectives
Willow leaf rust caused by Melampsora spp. is a serious disease on Salix species grown in short-rotation plantations. To devise an efficient control strategy, information on the Melampsora population genetic structure is required. We have started developing AFLP markers, a DNA fingerprinting technique based on the selective amplification of restriction fragments [1], to assess the genetic variation in rust samples collected from the same willow clones grown in three different localities in central and southern Sweden.

Results and conclusions
Preliminary results indicate a high degree of polymorphism. This genetic polymorphism will be used to study genetic diversity and to derive indirect estimates of migration rates. Current results will be presented.

References
1. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Homes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M, 1995. Nucleic Acids Research 23, 4407-4414.