DEVELOPMENT OF GENETIC MARKERS FOR RECONSTRUCTING PHYLOGENIES OF FUNGAL POPULATIONS AND TESTING FOR REPRODUCTIVE MODE
University of Copenhagen, Botanical Institute, Copenhagen, Denmark
Background and objectives
Genetic markers from known areas in the genome are useful in population genetic studies fungi. The characters can be visualized by PCR-SSCP, which makes it possible to screen a large number of loci and isolates. The technique makes it possible to assure homology of the detected alleles by direct sequencing.The data can be used to reconstruct further the phylogeny of the population making it possible to estimate the mode reproduction and detect epidemic subpopulations.
Materials and methods
We used 10 polymorphic loci in a population genetic analysis of Penicillium scabrosum. The genotypes were detected by SSCP and confirmed by sequencing.
Results and conclusions
The phylogenetic reconstruction and tests for reproductive mode showed that the population was recombining, allthough no sexual structures (teleomorph) of P. ;scabrosum are known.