MICRO FUNGI FROM SURFACE SOIL OF MULBERRY FARMS IN THE KASHMIR VALLEY AND THEIR POPULATION DYNAMICS.
MICRO FUNGI FROM SURFACE SOIL OF MULBERRY FARMS IN THE KASHMIR VALLEY AND THEIR POPULATION DYNAMICS. N.A.MIR AND N.A.MUNSIU DIVISION OF SERICULTURE S.K.A.U. S. T MIRGUND KASHMIR INDIA Background and objectives Many pathogenic fungi of mulberry are perpetuating in plant debris or directly in different morphological forms in soil. The purpose of the work reported here was to qualify and quantity, population of pathogenic and non pathogenic fungi from surface soils of various mulberry farms of the kashmir valley under single tillage. Materials and methods Soil samples were collected by means of sterile metal sampling tubes (Eicker, 1969). Composite soil samples of 17 mulberry farms(2-3 from each of the six districts of state). consisting of pooted soil of ten random samples , collected between plant rows up to depth of 15cnis, were dried for 24 hours at 28 degree celsius 1, after drawing the samples for moisture% & pH. The dried soil was sieved through 20 mesh screen and material retained on screen termed as plant debris , what passed through screen labelled as soil. Micro fungi were isolated from both the fractions. Both dilution plate method (Waksnian & Fred 1922) and soil plate method (Warcup,1950) were used to isolate & quantitate fungi from soil. In dilution plate method dilutions were made with 2% agar water. The medium used was potato dextrose agar -with traces of rose bengal or streptopencillin. In soil plate method 20mgs of soil were sprinkled per plate on Czapek's dox agar medium with 0.5% yeast extract and traces of rose bengal or streptopencillin. Isolation for pure culture were made on potato dextrose agar, Czapek's dox-agar & Richard's agar media. Fungi obtained from plant debris and soil were identified employing standard techniques. The identification were got confirmed where ever needed from M (England) and ITIC (IARI) New Delhi. Results and conclusions Eight different fungi were found associated with plant debris which include: -Phleospora maculans, Altemaria tenuissima, Fusarium pallidoroseum, Camarosporiuni perssini, Tricothecium roseum, Helicobasidium campactum