TAXONOMY AND ECOLOGY OF THE PEZICULA CINNAMOMEA COMPLEX
GJM VERKLEY1, K SCHRADER2 and R DE
1Centraalbureau voor Schimmelcultures, PO Box 273, NL-3740 AG Baarn, The Netherlands; 2Institut for Mikrobiologie, TU Braunschweig, Spielmannstrasse 7, D-38106 Braunschweig, Germany
Backgrounds and objectives
Pezicula Tul. & C. Tul. is a genus of inoperculate discomycetes (Dermateaceae, Leotiales) with coelomycetous anamorphs in Cryptosporiopsis Bubik & Kabit and Phlyctema Desm. The common bark endophytes in the P. ;cinnamomea complex are insufficiently characterized and delimited. Traditionally, species are identified according to the host: deciduous trees for Pezicula cinnamomea (DC.) Sacc., and conifers for P. ;livida (Berk. & Br.) Rehm . Recent studies have shown that isolates belonging to a single species are present in the bark of both conifers and deciduous trees . We are aiming at a world revision of Pezicula.
Material and methods
Fresh fruitbodies were collected on various hosts in Europe and North America. Ascospore isolates were characterized morphologically, physiologically, and genetically by RFLP of PCR-amplified nuclear small subunit and ITS +5.8S regions of the ribosomal DNA, using six restriction enzymes.
Results and discussion
Our results indicate that at least three species can be distinguished in the cinnamomea complex: P. ;cinnamomea (DC.) Sacc., fruiting on deciduous trees and conifers; P. ;eucrita P. Karst., fruiting on conifers only; and a third species fruiting mainly on conifers, but found also on Fagus (USA) and Amelanchier (Netherlands). P. ;cinnamomea and the third species have been found in association with bark necrosis. P. ;eucrita has never been found in association with bark disease. RFLP of the small subunit revealed several distinct groups in Pezicula. One group comprises the P. ;cinnamomea complex with identical patterns for six enzymes tested. Digestions of ITS regions with four enzymes supported the three species as presented above, while the other enzymes did not give informative results. To date, 20 synonyms of P. ;cinnamomea, including P. ;livida, and one synonym of P. ;eucrita have been found.
1. Dennis, RWG, 1973. Kew Bulletin 29, 157-179.
2. Kowalski T, Kehr RD, 1993. Sydowia 44, 137-168.<.p>