SUGARCANE STRIATE MOSAIC DISEASE: DEVELOPMENT OF A DIAGNOSTIC TEST
N THOMPSON1, Y CHOI2 and JW RANDLES1
1Department of Crop Protection, The University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia; 2Department of Plant Pathology, University of California, Riverside, California, 92521, USA
Background and objectives
The symptoms of ScSMD include characteristic short chlorotic striations present on most leaves of the sugarcane plant. Other symptoms include a general yellowing, reduced growth rate, shorter internode length and less thickening of the cane. Symptoms of ScSMD become more severe for subsequent ratoons, with older ratoons showing dramatically reduced growth rate and severe symptoms. Diseased cane is usually ploughed out after the first ratoon because the reduced growth rate and other ScSMD symptoms make it uneconomical.
Diagnosis of disease currently depends on recognition of the symptoms described above, but as the severity of symptoms can vary between different varieties, time of year, and other environmental factors, disease diagnosis is unreliable.
ScSMD was recently suggested to have a viral etiology and a slightly flexuous rod-shaped virus-like particle with single stranded RNA was isolated from diseased cane and named sugarcane striate mosaic virus (ScSMV) . The RNA was partially sequenced . This paper describes the use of sequence data to develop molecular diagnostic methods for ScSMV so that etiology and epidemiology can be fully described.
Materials and methods
Results and conclusions