TRANSFORMATION OF DIFFERENT WHEAT VARIETIES
M CLAUSEN, J FUETTERER, C ZHANG, T ZHAO, I. POTRYKUS and C SAUTTER
Institute of Plant Sciences, ETH Zurich, Universitaetsstrasse 2, 8096 Zurich, Switzerland
Background and objectives
Results and conclusions
Our second approach concerned the expression pattern of the maize ubiquitin promoter. Promoter-GUS fusions were used to analyse in detail the tissue specific expression pattern of the promoter. Analysing various transgenic BobWhite lines we obtained a strong GUS expression in fast diving cells. High levels of GUS expression were obtained in roots and young meristematic tissue like the vegetative and floral mersitems. Since mature leaves showed very little to no GUS expression, we are questioning its application with respect to foliage diseases.
During the course of these experiments we accelerated the transformation methods such that today's regenerating shoots can be obtained in less than three months, reducing the danger of somaclonal variation.
Finally, various Swiss breeding varieties including Frisal, Golin, Greina and Attila, which are particularly susceptible to different fungal diseases, were transformed. As agronomically important traits we used genes involved in horizontal resistance, for example the wheat thaumatin-like gene or genes which play an important role in more specific plant resistances. We will discuss in detail our results on some of these transgenic lines.