PROTEIN PATTERN IN CELL SUSPENSION CULTURES OF ULMUS PUMILA AND U. CAMPESTRIS INOCULATED WITH SPORES OF OPHIOSTOMA ULMI
MJ BABIANO, R MANSO, T VALLE and P CORCHETE
Dpto Fisiologia Vegetal, Facultades de Biologia y Farmacia, Universidad de Salarnanca, Spain
Background and objectives
When cell suspension cultures derived from leaf-callus of Ulmus pumila (an elm species resistant to DED) or U. campestris (a high susceptible elm) were inoculated with O. ulmi spores, mycelial growth was visible 24 h after inoculation in U. campestris . By contrast no fungal growth was observed in Ulmus pumila cultures after 4 days of inoculation. This differential growth on "in vitro" cultured elms reflect a degree of resistance of the Ulmus pumila tissue from which the cultures were derived . These cultures are being used as a model system to study the differential biochemical mechanisms of disease resistance in elms. In this work we have studied the protein pattern in both resistant and susceptible elm cell cultures and the alterations brought about by the infection with spores of 0. ulmi .
Results and conclusions