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INSERTIONAL MUTAGENESIS IN STAGONOSPORA NODORUM,
INSERTIONAL MUTAGENESIS IN STAGONOSPORA NODORUM, CLARE RUSHOWSKIl, NEIL COOLEY 2, and PAUL BOWYERl. l Long Ashton Research Station, Long Ashton, Bristol. BS1 8 9AF. UK; 2AgrEvo UK Ltd, Chesterford Park, Saffron Waiden, Essex. CB1 1 1XL. UK The phytopathogenic fungus Stagonospora (Septoria) nodorum is the causative agent of leaf and giume blotch in wheat. It is one of the most important crop pathogens in the UK, and also occurs world-wide. As a nectrotrophic leaf pathogen, it causes losses through reduced growth of leaves and through the infection and destruction of developing grain. Various approaches can be used to study fungal pathogenicity genes. Reverse genetics can be used, but only in systems where pathogenicity related proteins have been identified. Alternatively RNA-based approaches can be used, although these cannot distinguish between genes required for, and those expressed during, pathogenicity. The third option, used in this work, is mutagenesis. We are using REMI, a form of insertional mutagenesis, to look for genes involved in pathogenicity in S.nodorum
  • . So far we have successfully generated approximately 800 transformants, and these are being examined for auxotrophy and pathogenicity.