6.43
RED LEAF MOTTLE DISEASE OF SUGARCANE: NEW RECORD FROM INDIA

PP UPADHYAYA1 and GP RAO2

1Department of Botany, DDU Gorakhpur University, Gorakhpur 273 009, UP, India; 2Sugarcane Research Station, Kunraghat, Gorakhpur 273 008, UP, India

Background and objectives
In India, the sugarcane crop has been reported to be affected by many viruses: sugarcane mosaic virus, sorghum mosaic virus and sugarcane bacilliform virus [1]. In recent years, however, many new virus diseases of sugarcane have been reported from abroad [2], whose existence in India is still unknown. These virus diseases are causing alarming losses to the sugarcane crop and to sugar recovery. An attempt was made to characterize an unidentified virus disease occurring on sugarcane crop in India.

Materials and methods
DAS-ELISA and Western blotting techniques were adopted for checking serological relationships of the virus under study.

Results and conclusions
During a survey of sugarcane crops in 1997-98 in the vicinity of Gorakhpur, India, a range of symptoms, consisting of small diamond-shaped chlorotic spots with narrow to broad bands along the midrib of matured leaves, was observed in varieties CoSe 93232 and 92423. The spots later turned red and the leaves developed a red mottling on a large area of the leaf lamina. The variety CoSe 93232 also exhibited long, red, interrupted stripes on young leaves. Leaf-dip preparations of the infected sample showed the presence of rod-shaped particles of size 260x20 Ám under the electron microscope. The incidence of the disease varied from 2-7% in different fields. The virus was identified as peanut clump virus on the basis of symptomatology, particle morphology and serological relationship by DAS-ELISA and Western blotting with a French isolate of peanut clump virus. This is the first report of this disease from India.

References
1. Rao GP, 1996. Botanica 49, 220-25.
2. Frison EA, Putter CAJ, 1993. FAO/IBPGR Technical Guidelines for the safe movement of Sugarcane Germplasm. FAO, Rome/IBPGR, Rome.