Figure 2. Nucleotide sequence and deduced amino acid sequence of PtrNEC. The portion of the amino acid sequence confirmed by protein sequencing is shown in purple. The single internal SalI and HindIII sites (green) along with the main and in-frame secondary stop codons (blue) are highlighted. Location of putative leader peptide (M1-A16) and potential N-termini of the mature toxin are marked in orange.