mppol/1997/0123uyeda Figure 3


Figure 3. Distribution in CsTFA density gradient centrifugations. Core particles were subject to CsTFA centrifugation in (A) 2.35 M, (B) 3.57 M CsTFA in 1 mM DTT solution. (a) Aliquots were analysed by 7.5 % SDS-PAGE and stained with silver. (b) Aliquots were analysed by 1 % agarose gel electrophoresis and stained with 0.5 ug/ml ethidium bromide and visualised under UV light. Open core particles were identified in fractions (A)9-11. Protein-free genomic dsRNAs were identified in fractions (B)2-4. P7-dsRNA complexes were identified in fractions (B)5-13. Purified virus was loaded in the V lane as a marker.