Figure 2. SDS-PAGE of proteins secreted by Oomycetes in liquid culture.
Culture filtrates from Pythium vexans (Py. vex.), Pythium ostracodes (Py. ost.), Pythium graminicola (Py. gra.), Pythium myriotylum (Py. myr.), Pythium arrhenomanes (Py. arr.), Achlya ambisexualis (Ac. amb.), Pachymetra chaunorhiza (Pa. cha.), Phytophthora melonis (Ph. mel.), Phytophthora syringae (Ph. syr.), Phytophthora clandestina (Ph. cla.), and Phytophthora erythroseptica (Ph. ery.) were electrophoresed alongside purified Beta-cryptogein from Phytophthora cryptogea (Ph. cry.) and molecular weight standards (S). Proteins were stained with Coomassie Blue without prior fixation. Molecular weights (Mr x 10-4) are marked. E marks the expected mobility for elicitins.