Figure 1. Screeing for L. maculans-inducible clones. In the example,
duplicate filters were spotted with equal volumes of PCR-derived cDNA inserts
(see Methods for details). Filters were hybridized using DIG-labeled cDNA
populations from either uninoculated or L. maculans-inoculated B.
napus leaves. Examples of cDNAs hybridizing more strongly with probe
from fungal-inoculated leaves are circled. As controls, all filters included
the following cDNA inserts: DH27-11(GenBank AA960723)
encoding phenylalanine ammonia lyase (PAL), DH28-11 (AA960724),
encoding the
Lhcb1 chlorophyll a/b binding protein (Cab), and the
B. napus PR1 cDNA (U64806).
Fristensky et al.