Molecular Plant Pathology On-Line [http://www.bspp.org.uk/mppol/1997/0220sivamani]

Cloning and In Vitro Expression of the Wheat Streak Mosaic Virus NIb Gene.

# Sivamani E, $ Qu R.

# Department of Plant, Soil and Environmental Sciences, Montana State University, Bozeman, Montana, MT 59717-0312, USA.

$ Department of Crop Science, Box 7620, North Carolina State University, Raleigh, NC 27695-7620.

Corresponding author:

R. Qu, Department of Crop Science, Box 7620, North Carolina State University, Raleigh, NC 27695-7620.
Telephone: 919-515-7616 Fax 919-515-7959 email: rongda_qu@ncsu.edu

ABSTRACT

Recombinant plasmids containing cDNA from the wheat streak mosaic virus (WSMV) isolated from Montana were constructed and nucleotide sequences were determined. A 1485 nucleotide sequence containing the putative full-length nuclear inclusion protein b (NIb) and flanking regions was further studied. The deduced amino acid sequence of the NIb gene was compared to homologues from two aphid transmitted (PVY and TEV) and one mite transmitted (BrSMV) potyviruses. The WSMV-NIb protein showed 44-66% identity with corresponding proteins of the other potyviruses, with the highest degree of conservation with the mite transmitted BrSMV. The predicted WSMV-NIb protein product is 48.3 kDa. However, the WSMV-NIb cDNA in vitro translation product migrated at approximately 42 kDa.

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