Molecular Plant Pathology On-Line [http://www.bspp.org.uk/mppol/1997/1124pipe]

Telomere-associated restriction fragment length polymorphisms in Phytophthora infestans

1Pipe ND, 2Shaw, DS.

1bss215@bangor.ac.uk. 2d.s.shaw@bangor.ac.uk

University of Wales, Bangor, School of Biological Sciences, Bangor, Gwynedd, LL57 2UW, UK.

Corresponding author:

DS Shaw. University of Wales, Bangor, School of Biological Sciences, Bangor, Gwynedd, LL57 2UW, UK. Tel: +44 (0)1248 382541, Fax: +44 (0) 1248 370731, Email: d.s.shaw@bangor.ac.uk

ABSTRACT:

The probe pLT11, containing the telomeric DNA repeat (TTTAGGG)n from Arabidopsis thaliana hybridized to genomic DNA from Phytophthora infestans digested with HindIII. Fragments hybridizing to the telomeric DNA probe were sensitive to digestion with BAL31 confirming that they were telomere-containing end fragments. Hybridization of the telomeric probe to HindIII-MspI genomic fragments from P. infestans produced more than 24 bands. End-fragments were highly polymorphic between and within two populations. Isolates characterised as identical by the multi-locus probe RG57 had differing end-fragment profiles. The use of end-fragment length polymorphisms for fingerprinting isolates of P. infestans is discussed.