P. Jones ¹*, B.J. Devonshire¹, T.J. Holman¹ and S. Ajanga²

¹ Plant Pathogen Interactions Division, Rothamsted Research, Harpenden Herts. AL5 2JQ, UK
² KARI Kakamega, P.O. Box 169 Kakamega, Kenya

*phil.jones@bbsrc.ac.uk

Accepted for publication 19/03/04

Napier or Elephant grass (Pennisetum purpureum) is a native clumping grass of tropical Africa that grows 3 to 5 metres tall and is mainly propagated from cuttings of 3 to 4 nodes in length. It is widely used as a fodder crop and is also planted for environmental protection, to stabilise soils and act as windbreaks. In Kenya, it has been used in a novel ‘push-pull’ pest management system for cereal stem borers (Khan et al. 2001). 

Figure 1 Left: Napier grass stunt (foreground) and apparently healthy grass (rear) Right: yellowed and stunted clumps of Napier grass

Since 2000, symptoms have been seen on Napier grass in western Kenya that include foliar yellowing, little leaves, proliferation of tillers and shortening of internodes to the extent that clumps appear severely stunted. Tests for viruses and root-infecting fungi that might cause these symptoms were all negative. Leaf samples from both yellowed and apparently healthy Napier grass were sent to Rothamsted, as were rooted plants which were grown on under quarantine. Total DNA extracted from each leaf sample was used as template in a nested PCR using phytoplasma ribosomal DNA primer pairs P1/P7 and then R16F2n/R16R2. A 1250-bp rDNA product was amplified from all yellowed leaves and from two of three apparently healthy leaves. All plants grown-on had yellowed leaves and stunted growth and were phytoplasma positive. RFLP analysis of the rDNA amplimers showed the same pattern for all samples, so four samples were chosen for cloning and sequencing. Analysis of rDNA sequences (deposited in Genbank as Accessions AY377874-AY37787) identified these phytoplasmas as members of the 16Sr XI (rice yellow dwarf) group. Strains were most similar (96%) to Bermuda grass white leaf phytoplasma (Accession Y16388). No information is available about putative insect vectors but the potential for spread of the phytoplasma in this part of Africa, by clonal propagation of infected cuttings, is considerable.

Figure 2. Results of nested PCR. From left 1kb ladder (MBI); 1-11 individual plants of stunted Napier grass; water control (reamplified); positive phytoplasma control; water control (nested PCR); 1kb ladder

Khan ZR, Pickett JA, Wadhams LJ, Muyekho F, 2001. Habitat management strategies for the control of cereal stem borers and striga in maize in Kenya. Insect Science and its Application 21, 375-380.

The British Society for Plant Pathology