MPP paper details first use of CRISPR in an oomycete
26 Sep 2015
CRISPR is a powerful molecular technique that enables the targeted slicing of DNA molecules, which in turn enables the sliced DNA to be modified or replaced by recombination. It has been applied to many species including plants, animals and fungi to introduce targeted mutations into those organisms.
Molecular Plant Pathology has published a Technical Advance by Yufeng Fang and Brett M. Tyler on the “Efficient Disruption and Replacement of an Effector Gene in the Oomycete Phytophthora sojae using CRISPR/Cas9” The paper demonstrates the first use of CRISPR in an oomycete. This is of particular importance because molecular techniques such as gene knockouts and gene replacements, widely used in other organisms, are not possible in oomycetes. The successful application of CRISPR described by Fang and Tyler therefore opens up a whole new area of research capability to the oomycete community.
Image: Cas9 and guide RNA constructs for Phytophthora sojae genome editing