The treated material is designated M0. The progeny of that is the M1, which is heterozygous. As the majority of mutations are recessive (i.e. defect) one screens second generation, self-crossed material: M2. This material is homozygotic for any mutated genes. Analogous to F.
Adapted from: Tronsmo, A.M., Collinge, D.B., Djurle, A., Munk, L., Yuen, J. and. Tronsmo, A. 2020 Plant pathology and plant diseases. CABI, Wallingford.
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